Effect of Chloramphenicol on Light-Dependent Synthesis of Proteins and Enzymes of Leaves and Chloroplasts of Phaseolus vulgaris.

Etiolated leaves of higher plants acquire the ability to photosynthesize during prolonged irradiation. At the same time, profound morphological and chemical changes occur in the leaf plastids (10). Among these are qualitative and quantitative changes in protein content. Percent protein contained in the plastid fraction of Cichorium (6) and protein content per plastid of Phaseolus (27) increases on prolonged irradiation of etiolated leaves. Increases also occur in TPN glyceraldehyde-3-P dehydrogenase (3, 11), PPNR4, transhydrogenase (20), ribulose-1,5-di P carboxylase (12), and alkaline fructose-1,6-diphosphatase (33). In contrast, synthesis of cytoplasmic DPN glyceraldehyde-3-P dehydrogenase is not dependent on light (3, 11). Evidence was presented in a previous paper that insoluble chloroplast enzymes necessary for light-dependent electron transport also might be formed in response to irradiation of etiolated leaves (25).